Congrats to Subhas, Pim and colleagues to put this preprint together! We used magnetic tweezers to monitor the bacterial open complex formation, stabilization and disruption in different salt and temperature conditions. We discussed several kinetic models and reveal that the RNAP dissociates from the intermediate open state. Furthermore, we show the importance of using the “right” monovalent salt, i.e. potassium glutamate, to efficiently form a stable open complex. A great collaboration with Anssi Malinen lab in Turku!
David Dulin lab 